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家兎子宮筋에서 分離한 Microsome 分劃內 Adenosinetriphosphatase 活性度에 미치는 Mg^(++), Ca^(++), Na^(+), K^(+) 및 Creatine phosphate의 影響

The Effect of Mg^(++), Ca^(++), Na^(+), K^(+) and Creatine Phosphate on the ATPase Activity of Microsomal Fraction from Rabbit Uterus

대한약리학잡지 1966년 2권 2호 p.35 ~ 42
최신정,
소속 상세정보
최신정 (  ) - 부산대학교 의과대학 약리학교실

Abstract


The author investigated the effect of Mg^(??), Ca^(??), Na^(+), K^(+) and creatine phosphate on the ATPase activity of microsomal fraction isolated from rabbit uterus and obtained the following results:
1) The uterine microsomal fraction contained the Na^(+)- and K^(+)- activated ATPase in the presence of Mg^(??). The ATPase activity increased with protein content in the fraction.
2) The maximum ATPase activity was obtained at Na^(+) and K^(+) concentraction of 100 mM respectively.
3) In the absence of Mg^(??), the ATPase was not activated by Na^(+) and K^(+), but inhibited.
4) Ca^(??) stimulated the Na^(+)- and K^(+)- activated ATPase in the presence of Mg^(??). However, in the absence of Mg^(??), the ATPase was not activated by Ca^(??).
5) The K^(+)- activated ATPase activity was greater than the Na^(+)- activated ATPase under all conditions.
6) The Na^(+)- and K^(+) activated ATPase activity was increased by addition of creatine phosphokinase and creatine phosphate to the reaction mixture.

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