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電氣泳動法 및 等電集焦法 (Isoelectric focusing)을 이용한 膵蛋白 分離實驗

대한의학협회지 1972년 15권 9호 p.63 ~ 71
金源准, 金景煥, 김응권,
소속 상세정보
金源准 (  ) 
연세대학교 의과대학 약리학교실

金景煥 (  ) 
연세대학교 의과대학 약리학교실
김응권 (  ) 
연세대학교 의과대학 약리학교실

Abstract


Electrophoresis is one of the electrokinetic phe-
nomera which involves the motion of dissolved
or suspended material under the influence of elec-
trio field.
In 1892 Picton and Linder employed V-and
U-shaped glass tube to observe the migration of
inorganic compounds and hemoglobin in solution.
Michaelis devised U-tube. to determine the
ioelectric point of several enzyme. preparations
which _ were among the ´. original application of
free or moving boundary electrophoresis reported
in detail by Tiselius in 1937.
Moving boundary electrophoresis is :extremely,
valuable for determination of ioelectric points.
but is usually difficult to maintain the bounda-
ries between separated fractions. Owing to its ex.
perimental- complexity and cost this technique
electrophoresis. The latter method uses a relati-
vely inert and homogeneous solid or semisolid me-
dium to ´hold the separated components in dis-
Crete zones for further study and it possesses the
greater sensitivity wish much smaller quantities
of sample.
Much of the earlier work involved the use of
filter paper as stabilizing medium. In recent ye-
ars, however, filter paper has been superseded
by other materials, such as cellulose acetate, star-
ch gel and polyacrylamide gel which permit
sharper separations. Particularly the -high resol-
Isoelectric Focusing in Polyacrylamide Gel acrylamide gel electrophoresis is to a large ex-
and its Application to Immunoglobulins, Nature, tent a consequence of molecular sieving acting as
219: 66,` 1968. - additional separative factor.
23) Carlstrom, A. and Vesterberg, O.: Isoelectric Fo-
casing. and Separation of the Subcomponents of Recently the relative new technique of isoelec- 3
Laetoperoxidase, Acta. Cheri. Scand., 21: 271, trio focusing, though not a electrophoresis in the
1967. = conventioi,al sense, were developed for separating
proteins, etc. for both analytical and preparati\Te j
.Studies on pancreatic enzyme-fractionation purposes.
by electrophoresis and ioelectric focusing. Isoelectric focusing involves the application of
a potential difference between the end of a col-
Electrophoresis is one of the electrokinetic phe- umn of electrolyte in which there is a pH gradient,
nomera which involves the motion of dissolved the anode being at the acid end. Each ampholyte

or suspended material under the influence of elec- present is driven towards and focused at the
trio field. position at which . it is ioelectric. Selection of
In 1892 Picton and Linder employed V-and appropriate pH range of ampholyte is one of
U-shaped glass tube to observe the migration of additional advantage..
inorganic compounds and hemoglobin in solution. In present study, we separated the pancreatic_
Michaelis devised U-tube. to determine the enzymes of rabbits by means of electrophoresis,
ioelectric point of several enzyme. preparations paper and disc, and isoelectric focusing.
which _ were among the ´. original application of The results obtained are compared and. sum:-
free or moving boundary electrophoresis reported marized as follows:
in detail by Tiselius in 1937. 1. By-means of paper electrophoresis the rabbit
Moving boundary electrophoresis is :extremely, pancreatic enzymes were separated into seven
-76" (794`
` Via,

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