海명螺旋神經細胞의 電子顯微鏡的 考察
Electron Microscopic Studies upon Normal Spiral Ganglion Cells of Guinea pigs
임장선,
소속 상세정보
임장선 ( Lim Jang-Sun ) - 연세대학교 의과대학 이비인후과학교실
KMID : 0361019700130040035
Abstract
Materials for these studies were taken from 6 healthy guinea pigs, 300 -350 gm in weight, which had revealed normal preyer s reflex. The modiolus and spiral lamina were exposed by careful removal of the bony capsule. The modiolus including cochlear nerve was brought immediately´ into 2% osmium tetraoxide adjusted at pH 7.4 with acetate veronal buffer. After fixation, they were dehydrated through a series of ethyl alcohol graded from :60%-100% for 20 minutes respectively.
After these procedures, bony portion of the modiolus and spiral lamina were trimming. Ultra-thin sections were cut from´Epon blocks by glass knives on :MT -2 Ultra-microtome, 400-600A think section and stained with saturated uracyl acetate solution and lead citrate solution for about 30 minutes and 15 minutes respectively. Electron-microscopic pictures of spiral ganglion cells were observed with Hitachi-He-11E-1 type electron microscope.
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