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이독성 난청에 대한 골수유래중간엽줄기세포의 치료 효과

Therapeutic Efficacy of Bone Marrow Derived Mesenchymal Stem Cells in Ototoxic Sensorineural Hearing Loss

대한이비인후과학회지-두경부외과학 2020년 63권 12호 p.564 ~ 569
김수빈, 권윤희, 김인범, 서영준, 한재상, 서재현, 이현진, 박경호,
소속 상세정보
김수빈 ( Kim Su-Bin ) - Catholic University College of Medicine Eunpyeong St. Mary’s Hospital Department of Otolaryngology-Head and Neck Surgery
권윤희 ( Kwon Yoon-Hee ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Otolaryngology-Head and Neck Surgery
김인범 ( Kim In-Beom ) - Catholic University College of Medicine Department of Anatomy
서영준 ( Seo Young-Jun ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Otolaryngology-Head and Neck Surgery
한재상 ( Han Jae-Sang ) - Catholic University College of Medicine Seoul St. Mary′s Hospital Department of Otorhinolaryngology-Head and Neck Surgery
서재현 ( Seo Jae-Hyun ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Otolaryngology-Head and Neck Surgery
이현진 ( Lee Hyun-Jin ) - Catholic University College of Medicine Incheon St. Mary’s Hospital Department of Otorhinolaryngology-Head and Neck Surgery
박경호 ( Park Kyoung-Ho ) - Catholic University College of Medicine Seoul St. Mary’s Hospital Department of Otolaryngology-Head and Neck Surgery

Abstract


Background and Objectives: Ototoxic sensorineural hearing loss causes permanent hearingloss in most cases. Recently there have been many reports describing cell base therapywith stem cells that has some effect on hearing recovery. We evaluated the efficacy of clinicalgrade, pre-made, human bone marrow derived mesenchymal stem cells (BM-MSCs) in ototoxicdeaf animal model.

Materials and Method : BM-MSCs were cultured in a clinical grade laboratory. The animalswere divided into 2 groups as follows: a saline injected control group and a stem cell injectedgroup (MSC-group). Cultured MSCs were transplanted into the brachial vein of the deaf micemodel. We recorded auditory brainstem response (ABR) and conducted immunohistochemistryat 1, 3, and 5 weeks.

Results : After the transplantation of MSC, a significant improvement in the hearing thresholdof ABR was observed in the MSC transplanted group. Five weeks after transplantation ofMSCs, hair cell regeneration was confirmed from the basal to the apex of the cochlea in fluorescentdyed image under the microscope compared to the control group.

Conclusion : BM-MSCs were effective in an acute ototoxic deaf animal model. These resultsshow that stem cell transplantation mediate inner ear regeneration.

키워드

Animal models; Cell therapy; Mesenchymal stem cells; Ototoxicity; Sensorineural hearing loss

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