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결절형 Tawa육종의 Collagenase에 관한 연구 Collagenolytic Activity of Solid Tawa Sarcoma

대한치과의사협회지 1973년 11권 8호 p.525 ~ 530
정태영, Sakaki Tetsuya, Tawa Toshikazu,
소속 상세정보
정태영 ( Chung Tai-Young ) 
서울대학교 치과대학 구강생화학교실

 ( Sakaki Tetsuya ) 
Japan Osaka Dental University
 ( Tawa Toshikazu ) 
Japan Osaka Dental University

Abstract


True collagenolytic enzymes in animal tissues were first demonstrated by Gross and Lapiere (1962), who showed the ability of such an enzyme in the culture medium of living explants of tadpole tissue to degrade a specific substrate of undenatured collagen under physiological conditions. Recently, tumor-associated collagenolytic activity has been demonstrated in human neoplasm and in ascites V Carcinoma. This investigation have been peforme to determine whether or not a collagen lytic enzyme could e found in isolated solid Tawa sarcoma of Donryu female rat obtained the culture medium. The results were as follows. 1. 11.5mg% of hydroxyproline contained in Donryu rat skin collagen, which was extracted by 0.5M acetic acid. 2. Cultivation of solid Tawa sarcoma tissues on reconstituted rat skin collagen gels showed lysis of adjacent gel after 18 hours, and much more extensive lysis after 5 days. 3. Collagen substrate was not attacked by the common proteolytic enzymes, trypsin, pepsin, and pronase.

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