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The proper concentrations of dextrose and lidocaine in regenerative injection therapy: in vitro study

Korean Journal of Pain 2021년 34권 1호 p.19 ~ 26
우민석, 박지영, Ok Seong-Ho, Park Mi-Yeong, 손주태, 조만석, 신일우, 김연아,
소속 상세정보
우민석 ( Woo Min-Seok ) - Gyeongsang National University Department of Convergence Medical Science
박지영 ( Park Ji-Young ) - Gyeongsang National University Changwon Hospital Department of Anesthesiology and Pain Medicine
 ( Ok Seong-Ho ) - Gyeongsang National University Changwon Hospital Department of Anesthesiology and Pain Medicine
 ( Park Mi-Yeong ) - Gyeongsang National University Changwon Hospital Department of Anesthesiology and Pain Medicine
손주태 ( Sohn Ju-Tae ) - Gyeongsang National University Hospital Department of Anesthesiology and Pain Medicine
조만석 ( Cho Man-Seok ) - Gyeongsang National University College of Medicine Department of Anesthesiology and Pain Medicine
신일우 ( Shin Il-Woo ) - Gyeongsang National University Hospital Department of Anesthesiology and Pain Medicine
김연아 ( Kim Yeon-A ) - Gyeongsang National University Changwon Hospital Department of Anesthesiology and Pain Medicine

Abstract


Background: Prolotherapy is a proliferation therapy as an alternative medicine. A combination of dextrose solution and lidocaine is usually used in prolotherapy. The concentrations of dextrose and lidocaine used in the clinical field are very high (dextrose 10%-25%, lidocaine 0.075%-1%). Several studies show about 1% dextrose and more than 0.2% lidocaine induced cell death in various cell types. We investigated the effects of low concentrations of dextrose and lidocaine in fibroblasts and suggest the optimal range of concentrations of dextrose and lidocaine in prolotherapy.

Methods: Various concentrations of dextrose and lidocaine were treated in NIH-3T3. Viability was examined with trypan blue exclusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Migration assay was performed for measuring the motile activity. Extracellular signal-regulated kinase (Erk) activation and protein expression of collagen I and α-smooth muscle actin (α-SMA) were determined with western blot analysis.

Results: The cell viability was decreased in concentrations of more than 5% dextrose and 0.1% lidocaine. However, in the concentrations 1% dextrose (D1) and 0.01% lidocaine (L0.01), fibroblasts proliferated mildly. The ability of migration in fibroblast was increased in the D1, L0.01, and D1 + L0.01 groups sequentially. D1 and L0.01 increased Erk activation and the expression of collagen I and α-SMA and D1 + L0.01 further increased. The inhibition of Erk activation suppressed fibroblast proliferation and the synthesis of collagen I.

Conclusions: D1, L0.01, and the combination of D1 and L0.01 induced fibroblast proliferation and increased collagen I synthesis via Erk activation.

키워드

Actins; Cell Migration Assay; Cell Proliferation; Collagen Type 1; Extracellular Signal-Regulated MAP Kinases; Fibroblast; Glucose; Lidocaine; Muscle; Smooth; Prolotherapy

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