잠시만 기다려 주세요. 로딩중입니다.

오가피청혈플러스가 NF-κB 및 MAPKs 신호전달체계와 혈관부착인자 등에 미치는 영향

The Effect of Acanthopanax sessiliflorum Cheonghyeol Plus on NF-κB and MAPKs Signaling and Vascular Attachment Factors

대한한방내과학회지 2020년 41권 6호 p.967 ~ 983
이지원, 최규철, 배지은, 유호룡, 김윤식, 설인찬,
소속 상세정보
이지원 ( Lee Ji-Won ) - Daejeon Jaseng Hospital of Korean Medicine Department of Korean Internal Medicine
최규철 ( Choi Gyu-Cheol ) - Daejeon Jaseng Hospital of Korean Medicine Department of Korean Internal Medicine
배지은 ( Bae Ji-Eun ) - Daejeon Jaseng Hospital of Korean Medicine Department of Korean Internal Medicine
유호룡 ( Yoo Ho-Ryong ) - Daejeon University College of Korean Medicine Department of Cardiology and Neurology of Korean Medicine
김윤식 ( Kim Yoon-Sik ) - Daejeon University College of Korean Medicine Department of Cardiology and Neurology of Korean Medicine
설인찬 ( Seol In-Chan ) - Daejeon University College of Korean Medicine Department of Cardiology and Neurology of Korean Medicine

Abstract


Objective: This study was performed to investigate the effect of Acanthopanax sessiliflorum Cheonghyeol plus (ASCP) on NF-κB and MAPK signaling and vascular adhesion factors associated with dyslipidemia in human umbilical vein endothelial cells (HUVECs).

Methods: We measured the scavenging activity of DPPH radical and ABTS radical by ASCP in HUVECs. We measured the protein expression levels of NF-κB, IκBα, ERK, JNK, and p38 after treatment of HUVECs with TNF-α. We measured the expression levels of MCP-1, ICAM-1, and VCAM-1 mRNA and of MCP-1, ICAM-1, and VCAM-1 biomarkers after treatment of HUVECs with TNF-α.

Results: The DPPH and ABTS radical scavenging activity of ASCP increased in a concentration-dependent manner. NF-κB, IκB, ERK, p38 protein expression levels decreased following ASCP treatment at all concentrations compared to untreated control HUVECs. JNK protein expression levels decreased in ASCP-treated HUVECs compared to untreated controls at concentrations of 100 μg/mL. MCP-1 mRNA expression level decreased with ASCP treatment ≥200 μg/mL compared to the control. ICAM-1 and VCAM-1 mRNA expression levels decreased at all concentrations compared to the control. MCP-1 protein expression level was reduced compared to the control at concentrations ≥200 μg/mL, ICAM-1 protein expression level was reduced compared to the control at concentrations ≥100 μg/mL, and VCAM-1 protein expression level was reduced at all concentrations.

Conclusions: These results suggest that ASCP has an antioxidative and hypolipidemic effect and that ASCP could treat and prevent dyslipidemia, atherosclerosis, and cardio-cerebrovascular diseases.

키워드

Acanthopanax sessiliflorum; Cheonghyeol-Plus; dyslipidemia; HUVECs; antioxidation activity

원문 및 링크아웃 정보

 

등재저널 정보