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세균을 이용한 CP 약침의 복귀돌연변이 시험

Bacterial Reverse Mutation Test of CP pharmacopunture

대한한의학회지 2020년 41권 3호 p.55 ~ 65
황지혜, 구자승, 정철,
소속 상세정보
황지혜 ( Hwang Ji-Hye ) - Gachon University College of Korean Medicine Department of Acupuncture & Moxibustion Medicine
구자승 ( Ku Ja-Seung ) - Bogwang Korean Medical Clinic
정철 ( Jung Chul ) - Namsangcheon Korean Medicine Clinic

Abstract


Objectives: This study aimed to evaluate the toxicity of CP pharmacopunture using bacterial reverse mutation test.
Methods: To determine the mutagenic potential of CP pharmacopunture, histidine requiring Salmonella typhimurium (TA98, TA100, TA1535, and TA1537) and tryptophan requiring Escherichia coli (WP2uvrA, pKM101) strains were used. The negative (normal saline solution) and positive (Sodium azide, 2-Nitrofluorene, 2-Aminoanthracene, 9-Aminoacridine, and 4-Nitroquinoline N-oxide) control groups were used. To determine the dose levels of the main study, a dose range-finding study was conducted.

Results: As a results of the dose range-finding study, the growth inhibition by CP pharmacopunture was not evident at any dose levels in the absence and presence of metabolic activation. As a results of the main study, the mean number of revertant colonies was less than twice when compared to the negative control values at all dose levels of the CP pharmacopuncture in the presence and absence of metabolic activation, showing no dose-related increase. In the positive control group, the number of revertant colonies was markedly increased by more than twice when compared to the negative control group.

Conclusion: According to the results of this study, CP pharmacopunture did not show any signs of mutagenic potential.

키워드

CP pharmacopunture; capsaicin; toxicity; bacterial reverse mutation test; acupuncture

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