잠시만 기다려 주세요. 로딩중입니다.

Modulation of Stress Protein Gene Expression by Environmental Stress and pH in the Mouse Fibroblasts and SCK Tumor Cells

한국동물학회지 1985년 28권 2호 p.108 ~ 119
강만식, 이정주, 이봉근, 서미영,
소속 상세정보
강만식 ( Kang Man-Sik ) - 서울대학교 자연과학대학 동물학과
이정주 ( Lee Chung-Choo ) - 서울대학교 자연과학대학 동물학과
이봉근 ( Lee Bong-Geun ) - 서울대학교 자연과학대학 동물학과
서미영 ( Suh Mi-Young ) - 서울대학교 자연과학대학 동물학과

Abstract


Aimed at elucidating the modulation of stress protein gene expression, the effect of environmental stress and pH on the induction of stress protein synthesis has been analyzed using SDS-polyacrylamide gel electrophoresis. Although the general patterns of protein synthesis in MEF and SCK cells are different, stress protein patterns are identical in both cells. Among three stress proteins, the $SP_70$ exhibits an interesting kinetics of induction and decay. The kinetics of $SP_70$ under acidic or normal pH appears to be similar, but the degree of hyperthermia and duration of treatment required for maximum induction are found to be different, being lower temperatures and shorter durations under acidic pH compared to those under normal pH. Inducation of stress protein and the accumulation of mRNA coding for stress proteins are blocked with actinomycin D, indicating the new RNA transcription is required for stress blocked with actinomycin D, indicating that new RNA transcription is required for stress protein induction. Treatment of cycloheximide during the after hyperthermia indicates that no specific protein is required for the induction of stress protein synthesis. Based on our preliminary data, we postulate that induction of stress protein synthesis in MEF and SCK cells is regulated primarily at the level of transcription and that $SP_70$ autoregulates its synthesis and levels of this protein are correlated with the stresseed state of a cell.

키워드

원문 및 링크아웃 정보

등재저널 정보