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Affinity Purification of Human Alpha-Fetoprotein

한국동물학회지 1986년 29권 4호 p.283 ~ 293
강신성, 방옥선, 박대규,
소속 상세정보
강신성 ( Kang Shin-Sung ) - 경북대학교 생물학과
방옥선 ( Bang Ok-Sun ) - 경북대학교 생물학과
박대규 ( Park Tae-Kyu ) - 경북대학교 생물학과

Abstract


For the preliminary step to make and characterize the monoclonal antibodies of human alpha-fetoprotein (AFP) was purified from 534g of human fetal tissues through the procedures of tissue extraction, DEAE-cellulose, concanavalin A-Sepharose, Cibacron Blue F3GA-agarose and immunoadsorbent affinity chromatography. The isolated AFP preparation showed a single band on polyacrylamide gel electrophoresis and a single precipitin are against rabbit anti-human cord serum and anti-human AFP on immunoelectrophoresis. Our AFP also displayed a single band on SDS-polyacrylamide gel electrophoresis. The recovery of AFP was 8.76mg total.

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