잠시만 기다려 주세요. 로딩중입니다.

배양계배 근세포의 분화과정에 미치는 중금속 이온의 영향

The Egect of Heavy Metal tons on the Differentiation of Cultured Muscle Cells of Chick Embryo

한국동물학회지 1987년 30권 4호 p.410 ~ 416
위인선, 이종빈,
소속 상세정보
위인선 ( Wui In-Sun ) - 전남대학교 자연과학대학 생물학과
이종빈 ( Lee Jong-Bin ) - 전남대학교 자연과학대학 생물학과

Abstract


The effect of heavy metal ions on the synthesis of proteins in cultured chick embryonic muscle cells were examined by labeling the cellular proteins with 35S-methionine and the surface proteins with Nalssl and lactoperokidase. The protein pattern in the cells cultured for 48 hrs showed little or no difference whether or not the cells were treated with any of the metal ions including Cu2+, Cd2+ and Hg2+, which are known to block the fusion of mypblasts. However, a 43kd protein disappeared from the control cells cultured for 72 hrs but remained unchanged in the cells treated with the metal ions. When analyzed for the syntheiic pattern of membrane proteins, addition of the ions (particularly of Cda+ and Cr3+) caused a marked increase in the level of 66kd protein, as compared to that in the untreated cells. By contrast, the level of 29kd protein was much higher in the control cells than in the cells treated with the metal ions. These results suggest that the heavy metal ions appear to block the degradation of 43kd soluble protein and 66kd membrane protein, perhaps by inhibiting a metalloprotease, which may be essential for the myogenic process of embryonic muscle cells.

키워드

원문 및 링크아웃 정보

등재저널 정보