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북방산 개구리(Rana dybowskii)의포의 프로젝트론 생서에 대한 cAMP의 조절작용

Role of cAMP in the Regulation of Progesterone Production and Secretion by Frog (Rana dybowskii) Follicles in vitro

한국동물학회지 1988년 31권 3호 p.177 ~ 184
권혁방, 안연섭, 김지열, 윤용달,
소속 상세정보
권혁방 ( Kwon Hyuk-Bang ) - 전남대학교 자연과학대학 생물학과
안연섭 ( Ahn Ryun-Sup ) - 전남대학교 자연과학대학 생물학과
김지열 ( Kim Ji-Yeul ) - 전남대학교 의과대학 핵의학교실
윤용달 ( Yoon Yong-Dal ) - 한양대학교 자연과학대학 생물학과

Abstract


The pattern of progesterone production and secretion of frog(R. dybowskii) follicles was investigated in follicle culture in vitro. Involvement of cAMP in the regulation of the steroid production by the follicles was also investigated by manipulating endogeneous cAMP level with forskolin and/or 3-isoburyl- 1 - methylxanthine(IBMX). Endogeneous follicular progesterone level increased rapidly in one hour of culture by treatment of frog pituitary homogenate(FPH) and reached peak level at 2 hours or later. But the absolute amount of progesterone produced (60-300 pg/follicle) or the peak time of the honnone level was different between individual animals. Basal level of progesterone in untreated sister follicles was very low (around 10 pg/follicle) and nearly undetectable in most cases regardless of culture lime. Secretion level of progesterone by the follicles obtained by measunng the honnone in the culture media was just the reflection of the intrafollicular level. Exogeneously added forskolin, an adenylate cyclase stimulator, and/or IBMX, a phosphodiesterase inhibitor, could mimic FPH action in terms of progesterone production and secretion. Thus, it seems clear that FPH regulates progesterone production via cAMP system in the follicle cells.

키워드

Folocular steroidogensis ; cAMP ; Amphibia

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