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천심련 물 추출물의 간세포보호효과

Hepatoprotective Effects of Andrographis Herba on t-BHP-induced HepG2 Cells

동서의학 2020년 45권 1호 p.41 ~ 47
정지윤, 박정아,
소속 상세정보
정지윤 ( Jung Ji-Yun ) - Daegu Haany University College of Korean Medicine
박정아 ( Park Chung-A ) - Daegu Haany University College of Korean Medicine

Abstract


Andrographis Herba is used as a traditional herbal medicine in the southeast Asian countries for the treatment of stomatitis, pharyngalgia, fever etc. In this study, we investigated the hepatoprotective effect of water extract of Andrographis Herba (AHE; the lyophilized yield was 11.2%) on tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in human hepatoma cell line HepG2. T-BHP is widely used to induce oxidative stress in a variety of cells. In HepG2, exposure to T-BHP results in excessive ROS generation and depletion of anti-oxidant systems. To investigate the hepatoprotective effect of AHE, cell viability was measured by MTT assay, apoptosis-related proteins, poly ADP ribose polymerase (PARP) and procaspase-3 were detected by immuno-blot assay and then analyzed the expression densities. In addition, the changes of H2O2 production was determined. Pre-treatment with two dosages of AHE (100 and 300 ㎍/㎖) significantly increased the cell viability, PARP and procaspase-3 reduced by t-BHP. In addition AHE also significantly decreased the oxidative stress in t-BHP-nduced HepG2 cells. These results suggest that AHE has hepatoprotective and anti-oxidant effect.

키워드

Andrographis Herba; hepatoprotective effect; PARP

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