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³²P-Labeled Chlorella의 정상배지에 있어서의 ³²P 및 total P의 전환

Turnover of ³²P and total P in ³²P-labeled Chlorella cells in a standard "cold" medium

미생물학회지 1966년 4권 1호 p.14 ~ 20
이영녹, 진평,
소속 상세정보
이영녹 (  ) - 고려대학교 이공대학 생물학과
진평 (  ) - 고려대학교 이공대학 생물학과

Abstract


1. Uniformly ³²P-labeled Chlorella cells were further grown in a standard "cold" medium and aliquots of the algal cells were taken out at the beginning of, and at intervals during the culture, and subjected to analyze the contents of ³²P and total P in various fractions of the cell constituents. 2. When the ³²P-labeled algae were grown in a normal "cold" medium, the P-contents in the fractions of DNA and protein increased. In the meantime the ³²P in acid-insoluble polyphosphate fraction decreased considerably, while that in RNA-polyphosphate complex significantly increased. 3. It was inferred that, under the experimental conditions of the present study, the phosphorus in polyphosphate seems to be transferred to RNA polyposphate complex and the phosphorus used in the synthesis of DNA and protein was, directly or indirectly, taken from those fractions above.

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