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미생물의 세포생리에 미치는 전이방사선의 영향에 관한 연구 3

Studies on the Cellular Metabolism in Microorganisms an Influenced by Gamma-irradiation (III); On the Changes ef Protein content and Free Amino anid Pool in Yeast cells irradiated by γ-ray

미생물학회지 1967년 5권 2호 p.79 ~ 85
김종협,
소속 상세정보
김종협 (  ) - 원자력연구소 생물학연구실

Abstract


Kim, Jong Hyup., (Div. of Biology, Atomic Energy Research Institute, Korea.;) Studies on the Cellulor Metabolism in Microorganisms as influenced by Gamma-irradiation(3): On the Changes of Free Amino acid Pool and content of Protein in Yeast clls irradiated by .gamma.-ray. 1. The strain of Saccharomyces cerevisiae had been cultured synchronously in aerobic condition and irradiatel by gamma-ray from the source of cobalt-60. Drying in vacuum oven at 90℃ over 12 hours, then changes of protein content (Kjeldahl) and free amino acid pool have been assayed with use of spectrophotometer. Results obtained were compared with those of unirradiated normal cells. 2. It is proved that amount of protein content in the irradiated cells increases to seven percent more than those of normal cells in the same weight of dried samples. It seems like carbohydrate breakown had been stimulated by irradiation and that relative contents of protein shows higher values than those of normal in the same weight of samples. 3. The amount of free amino acid pool in the irradiated cells shows less value about ten percent than those of normal cells, and rate of decreasing is also weak than those of standard reagent solution of amino acid. We may assume that free amino acid pool would be protected against radiation damage in living cells and more stable than in vitro. 4. The component of free amino acid pool have been assayed on second dimensional paper chromatogram, and the identified amino acids are as follows; aspartic acid, serine, glutamic acid, cystine, lysine, glycine, threonine, histidine, arginine, tyrosine, phenylalanine, valine and leucine. 5. Distributional presence of free amino acids are identical to that of normal cells except arginine, it is cosumable that radiation effect is univerlsal to all amino acid. However it is obvious that there are differences in radiolabilities of amino acids in irradiated cells.

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