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ºÓÀº »§°õÆÎÀÌ ¼¼Æ÷ÀÇ ´ç¿î¹Ý Sugar Transport in Conidia of Neurospora crassa

Korean Journal of Microbiology 1975³â 13±Ç 2È£ p.45 ~ 50
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Kyung Hee Univ. Department of Biology

Abstract

Û¡ä´µÇÁö ¾ÊÀº ºÓÀº»§°õÆÎÀÌ()NEurospora crassa)ÀÇ conidia°¡ ^(14)C-sorbose³ª ^(14)C-3-O-methylglucose¸¦ Èí¼öÇÏ´Â °ÍÀ» millipore filter technique·Î ÃøÁ¤ÇÏ¿´´Ù.

Sorbose³ª methylglucose°¡ á¬øàÒ®¿¡ ¿î¼ÛµÇ´Â ÃÖÀû pH´Â 4.75ÀÌ´Ù.

Û¡ä´µÇÁö ¾ÊÀº conidia¸¦ buffer¿ë¾×¿¡¼­ ±â¾Æ¸¦ ½ÃÄ×À» ¶§ Û¡ä´µÈ conidia³ª mycelia¿¡¼­ °üÂûµÈ °Í°ú´Â ´Þ¸® ±×µéÀÇ ÓØê¡áêÒöÕôÀº de-repressionÀÌ µÇÁö¾Ê°í Á¦2ÀÇ Á¶Àý±âÀÛÀ¸·Î½á ê¡ÚæµÇ°í ÀÖ´Ù.




Uptake of ^(I4)C-sorbose and ^(I4)C-3-¥Ï-methylglucose by ungerminated conidia of
Neurospora crassa was measured by means of the millipore filter technique. Initial rats
of uptake of both sorbose and 3-¥Ï-methylglucose show a marked dependence on pH of
the incubation medium in the range between pH 3.5 and pH 6.5. The optimal pH for
uptake of both sugars is close to 4.75.
When ungerminated conidia are "starved" with buffer for a prolonged period of time
prior to assaying their transport capacity and mycelia, no de-repression of the
glucose-repressible sugar transport system is effectuated in contrast to the findings for
germinated conidia.

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