Chlorelia의 生理에 미치는 2,4-Dichlorophenoxyacetic acid의 영향
Physiological Effects of 2,4-Dichlorophenoxyacetic acid(2,4-D) on Chlorella ellipsoidea
蔡麟基, 鄭英淑,
소속 상세정보
蔡麟基 ( )
梨花女子大學校 文理大學 生物學科
鄭英淑 ( )
梨花女子大學校 文理大學 生物學科
KMID : 0364819750130030101
Abstract
Chlorella의 生理에 미치는 2,4-D의 영향은 그 濃度에 따라 差異가 있었는데 그 結果는 아래와 같었다.
1) Chlorella의 生長은 2,4-D의 10^(-4)M농도에서 촉진되었고, 4×10^(-4)M에서 억제되었다.
2) 호흡은 생장촉진농도와 억제농도에서 모두 촉진되었고, 광합성은 생장촉진농도에서 촉진되었고, 억제농도에서는 control과 별차이가 없었다.
3) 대사물질중, 생장촉진농도에서 단백질, 핵산등은 증가되었는데, 탄수화물은 control에 비하여 별증감이 없었다. 그리고 억제농도에서는, 탄수화물, 단백질 및 핵산이 모두 감소되었다.
4) 결국 2,4-D에 의한 Chlorella의 생장촉진과 억제는 핵산과 그에 따른 단백질의 증감에 기인하는 것으로 짐작된다.
Physiological effects of 2.4-D on the growth of Chlorella ellipsoidea were investigated
culturing the algae in the MN₄media containing, 10^(-4)M and 4×10^(-4)M 2,4-D.
During 6 days culture of the algae, CO₂-enriched air was bubbled in under 10 K lux
of light at 25℃. At the given time intervals, samples of the culture were taken to
analysis with respect to overall growth, photosynthesis, respiration and chemical
composition. Results obtained from the experiment were as follows:
(1) The growth of Chlorella was increased at 10^(-4)M and decreased at 4×10^(-4)M of
2.4-D concentrations.
(2) At 10^(-4)M of 2,4-D concentration, the activity of photosynthesis enhanced relative
to control, while at 4×10^(-4)M it was not changed. In both concentractions however,
the rate of respiration was down from the control.
(3) At 10^(-4)M 2,4-D, the concentration of carbohydrate metabolites was not changed
relative to control, while significant increase in the concentrations of proteins and nucleic
acids was obseved. On the other hand at 4×10^(-4)M of 2,4-D concentrations, all the
metabolites including carbohydrates, proteins and nucleic acids were decreased.
(4) It is concluded that 2,4-D at 10^(-4)M concentration accelerates the growth of
Chlorella by promoting the activities of photosynthesis and biosynthesis of proteins and
nucleic acids.
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