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m-toluate를 분해하는 pseudomonas의 분리 및 degradative plasmid와의 연관성에 관하여 Studies on the m-toluate degradating plasmid in pseudomonas

Korean Journal of Microbiology 1979년 17권 1호 p.25 ~ 41
박순희, 하영칠, 홍순우,
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박순희 (  ) 
서울대학교 자연대학 미생물학과

하영칠 (  ) 
서울대학교 자연대학 미생물학과
홍순우 (  ) 
서울대학교 자연대학 미생물학과

Abstract


A strain able to grow up m-toluate minimal medium has been isolated after selective enrichment and given the name T81X, which was later identified as pseudomonase putida according to its morphological and biochemical characteristics. After treatment with plasmied specific curing agent, mitomycin C, followed by replica plating on m-toluate and xylene minimal agar plate, T81Xstrain has been shown to harbour a curable plasmid relating to the m-toluate and xylene metabolism. Spontaneous curing frequency of this plasmid was also greatly enhanced by growing on benzoate minimal medium. After then, it was also xylene metabogrowing on benzoate minimal medium. After then, it was found to be conjugally nontransmissible. From the comparative investigation of catechol 1,2-oxygenase and catechol 2,3-oxygenase activities in wild type and cured strain on various growth substrate, it appeared that T81X strain has both of these two enzymes while cured strain has catechol 1,2-oxygenase only. Growing on m-toluate minimal medium T81X strain should carry the genetic information necessary for coding the catechol 1,2-oxygense induced by m-toluate or benzoate, on that curable plasmid.

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