잠시만 기다려 주세요. 로딩중입니다.

분열효모에서 hrb1/SPAC328.05가 mRNA 방출에 미치는 영향

Effects of hrb1/SPAC328.05 on mRNA export in fission yeast

미생물학회지 2020년 56권 4호 p.356 ~ 360
김유경, 정민영, 윤진호,
소속 상세정보
김유경 ( Kim Yu-Kyung ) - Sungshin Women’s University Department of Biotechnology
정민영 ( Jeong Min-Young ) - Sungshin Women’s University Department of Biotechnology
윤진호 ( Yoon Jin-Ho ) - Sungshin Women’s University Department of Biotechnology

Abstract


In fission yeast, Schizosaccharomyces pombe, we previously reported the isolation of mutant (SLrsm1), which showed a synthetic lethality in combination with the rsm1 null allele, to identify genes necessary for mRNA export. Here, we demonstrate that the hrb1/SPAC328.05 gene is isolated, which partially suppresses the defects of growth and mRNA export of SLrsm1. This gene encodes an RRM (RNA recognition motif)-containing protein that is homologous to Saccharomyces cerevisiae Gbp2 and Hrb1 involved in mRNA surveillance and nuclear mRNA quality control. The deletion of hrb1/SPAC328.05 did not cause any defects of growth and mRNA export. However, its overexpression exhibited severe growth defect and accumulation of poly(A)+ RNA in the nucleus. Moreover, we observed that the Hrb1-GFP protein is localized predominantly in the nucleus. These observations support the possibility that the S. pombe Hrb1/SPAC328.05 protein is also involved in mRNA export and quality control.

키워드

Hrb1; Gbp2; mRNA export; S. pombe

원문 및 링크아웃 정보

등재저널 정보

KCI