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Penicillin Shock의 實驗的 硏究(第三報)

Studies on the Experimental Penicillin Shock (III )

부산의대잡지 1963년 3권 1호 p.21 ~ 28
양학도,
소속 상세정보
양학도 (  ) - 부산대학교 의과대학 미생물학교실

Abstract


As previously reported, penicillin antibody prepared by sensitizing rabbits with penicillin-eggalbumin-complex antigen could successfully be transferred passively to guinea pig skin. However, in vitro tests including hemagglutination reaction resulted in failure. The reports by many authors that positive hemagglutination reaction, although accompanied to a certain extent by nonspecific reactions, could occasionally be demonstrated in patient´s sera prompted the experiment to detect penicillin antibody in sensitized rabbit´s serum
The results are summarized as follows.
1) The specific penicillin antibody was detected in the sensitized rabbit´s serum when the trypsinized sheep erythrocytes sensitized with penicillin were used as antigens in the hemagglutination test. The specificity of the reaction was confirmed by the blocking test with crystalline penicillin.
2) A procedure to increase the sensitivity of hemagglutination test was devised by adding adequate amounts of heated bovine serum and vitreous body in the reacting medium.
3) Penicillin antibody as prepared by sensitizing rabbits in this experiment simulated the antibodies in allergic patients as revealed by positive passive transfer test with an exception that the former was more heat-stable.
4) It was assumed that the equivocal results by others in demonstrating penicillin antibody from patient´s sera could possibly be due to the technical differences in the preparation of antigens

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