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정상 및 Alloxan-당뇨병 토끼 각 조직의 C^(14)-1-포도당 및 C^(14)-6-포도당의 산화대사에 관한 연구

Oxidative Metabolism of C^(14)-1-glucose and C^(14)-6-glucose in Various Tissues of Normal and Alloxan Diabetic Rabbits

부산의대잡지 1966년 6권 1호 p.49 ~ 58
김영상,
소속 상세정보
김영상 (  ) - 서울대학교 의과대학 생리학교실

Abstract


Oxidative metabolic pathways of glucose were observed in various tissues i. E. , liver, renal cortex, brain and heart of normal and alloxan diabetic rabbits by incubating in C14-1 and C14-6-glucose medium.
Each tissues, normal and alloxan dabetic rabbits were divided into two groups (A&B) and homogenized in the mixture consisting of 5cc phosphate buffer, C14-1-glucose and C14-6-glucose were added and carrier glucose so as to maintain the 200mg % of glucose concentration. There after each tissue homogenate was incubated in the Da´bnuff metabolic shaker at 38°C for 3 hours. At the end of incubatio.i period gas sample and incubation medium were analyzed for the radioactivities, total CO2 production and glucose uptake rates. The following results were obtained.
1. Glucose consumption rates showed no difference regardless of the shift of labeling position of glucose carbon atom and decreased significantely in alloxan diabetic tissses as compared with the control.
2. In the alloxan diabetic heart, renal cortex and liver tissues- total CO2 production rates decreased to about two third of the normal.
3. In alloxan diabetic tissue homogenate, the rates of CO2 production derived from medium C14-glucose which calculated by multiplying total CO2 production rate with relative specific activities were decreased to ranged from two third to one fourth of control despite the brain remained unchanged.
4. In the brain tissue no inhibition of catabolic oxidation of glucose was observed in the alloxan diabetes.
5. The fractions of oxidative pathway via the Embden Myerhof pathway-Tricarboxylic acid cycle was obtained as ratio of relative specific activity of C´-6-glucose and of C14-1-giucose, were decreased in alloxan diabetic tissues as compared with the control group.
It is concluded that the inhibitory effects on the catabolic pathway of glucose in alloxan diabetic tissues
Were chiefly via the Embden Myerhof pathway-Tricarboxylic acid cycle than Hexose monophosphate path way. Possible explanations of these effects in each tissue were briefly discussed.

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