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담배의 바이러스 罹病葉과 健全葉에 있어서의 遊離아미노酸에 關한 定量的 硏究

Qualitative Study of Free Amino Acids in Healthy and Tobacco Mosaic Virus Diseased Tobacco Leaves.

한국식물학회지 1964년 7권 1호 p.1 ~ 4
이강업,
소속 상세정보
이강업 (  ) - 전남대학교 문리과대학

Abstract


LEE, Kang Up (Dept. of Biology, Chunnam National Univ.) Qualitative study of free amino acids in healthy and tobacoo mosaic virus diseased tobacco leaves (Nicotiana Tabacum L., White burley). Kor. Jour. Bot. Ⅶ (1): 1-4. 1964.
A comparative study of free amino acid content in healthy and virus diseased tobacco leaves was carried out by author throughout the growing season from June to November of 1963.
The methods of qualitative analysis of free amino acids applied in this experiment is followed by Moore and Stein. 1,2 Free amino acids determined in this experiment are shown in Fig. Ⅰ, Ⅱ and Table 1. As the figure and the table are shown, four more amino acids such as a spartic acid, glutamic acid, tyrosine and phenylalanine are detected in the hralthy leaves; these four additional amino acids in the healthy leaves are conspicuous. More quantities of asparagine and alanine are detected in the diseased leaves than the healthy leaves and more quantities of tryptophan is detected in the healthy leaves.
It is presumed that such amino acids as tyrosine and phenylalanine are decreased by the incooperation of free amino acid to TMV protein in the process of the process of the leaf protein metabolism which is caused by TMV-RNA trapping action in the diseased leaf protoplasm. It is thought that the decrease of asparagine and the increase of aspaitic acid in the healthy leaves are the results of in incooperation of NH_2, produced by the protein dissimilation in the diseased leaves, to asparic acid; it´s reaction is caused by the respiration of the diseased leaves accelerated by TMV attack. It is presumed, consequetly, that the check of the diseased tobacco leave growth is influenced by the reduction of such amino acids as tryptophane and glutamic acid, which reduction may be due to the abnormal protein metabolism and the action of certain enzyme caused by TMV attack on host protoplast.

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