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洋蘭의 生長點培養에 關한 硏究(Ⅱ)

Studies on the Mericlonal Protocorm of Orchid(Ⅱ)

한국식물학회지 1968년 11권 3호 p.23 ~ 30
Harn Chang-Yawl,
소속 상세정보
 ( Harn Chang-Yawl ) - Radiation Agriculture Research Institute

Abstract


Uging several varieties of Cymbidium, investigations were carried out to make clear how the protocormic tissue develops from the cultured explant. Enplant to be cultured were prepared in several ways: exclusively apical meristem, apical meristem dissected out with the basal part attached, axillary bud primordia in their initial stage of development, or apical or axillary bud dissected out as a whole etc.
It was observed that protocorms or protocormic tissues were developed from the explant´s meristematic tissues regardless of where these tissues were located. Apical meristem, leaf primordia, leaf axil, or internodal part of young bud turned easily protocormic, while the scaly leaves of axillary bud or stem tissue of mother shoot turned quickly brwonish and died away.
Both in axillary and apical bud explant alike, whether they were cultured whole or divided, some took quickly green color while others were slower, and some developed protocorms easily while others remained unchanged for months. Varietal difference as well as environmental factors seemed to be responsible for it. Further details should be clarified by histogenetical investigations.

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