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건강기능식품 공전 내 HPLC-UV 기반 아스타잔틴 분석법 개선 및 평가 Development and Evaluation of HPLC-UV Method for Astaxanthin in Health Functional Foods Code

약학회지 2023년 67권 5호 p.311 ~ 317
정유성, 신정훈, 최용석, 한상범, 이동규,
소속 상세정보
정유성 ( Jeong Yoo-Seong ) 

신정훈 ( Shin Jung-Hoon ) 

최용석 ( Choi Yong-Seok ) 

한상범 ( Han Sang-Beom ) 

이동규 ( Lee Dong-Kyu ) 

Abstract


The HFF (Health Functional Foods) Code provides protocols for the quantitative analysis of 68 types of HFFingredients. Astaxanthin is one of the HFF with antioxidant properties, preventing cell damage and reducing eye fatigue,becoming popular in the HFF market. To improve the astaxanthin assay protocol, we changed the LC condition from atertiary pump to a binary pump and optimized the mobile phase and gradient accordingly. Based on the differences in the enzymes, we also observed the efficiency of the conversion of astaxanthin ester to free astaxanthin. We validated ourmodified method based on AOAC guidelines, including linearity (R2?0.99), selectivity, accuracy (recovery 92~105%), andprecision (%RSD ?2). An applicability was evaluated on 15 health functional food products, and the recovery values weresatisfied within the range of 80-120%. In this study, we modernized the analytical method of astaxanthin by modifyingthe LC conditions, and standardized the cholesterol esterase with the highest efficiency.

키워드

Astaxanthin; Health Functional Foods (HFF) Code; Liquid chromatography; Ultraviolet detector

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