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外科患者에서 分離한 黃色 葡萄狀球菌의 L形의 誘發과 保存에 關하여

The Induction of L-Forms of Staphylococus aureus and Their Maintenance

대한외과학회지 1966년 8권 4호 p.221 ~ 223
배양태,
소속 상세정보
배양태 (  ) - 전남대학교 의과대학 외과학교실

Abstract


This experiment was undertaken in order to induce L-forms of some staphylococcus aureus strains which were not easily transformed to the L-form by conventional methods.
1) The most suitable concentration of methicillin to induce and maintain the L-form of staphylococcus aureus was formed to be microgram contained the media per one milliliter.
2) The staphylococcus aureus strain, which was isolated from a patient of (fatal) bacteremia could not be induced to L-forms by cultivating on the inducing media enriched with of human plasma but easily induced by cultivating on the media enriched with pregnant ewe´s plasma.
The L-form of strain I which could grow on the media of pregnant ewe´s plasma, but not grown on human plasma media started growing on the human plasma media after passages on the media of pregnant ewe´s plasma.
3) L-forms of strain Ⅱ, Ⅲ could easily reverted to their original coccal forms, when cultivated on methicillin free media. But after 32 passages as L-form, strain I could not be reverted to roccal form on antibiotic free media. Thus the longer the L-form maintained on the L-form media containing methicillin, the longer it would be to reverse to their original coccal form on antibiotic free media.
4) The role and mechanism of animal proteins for the induction of L-forms briefly discussed.

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