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遠沈法에 依하여 分離한 細胞成分의 癌成長에 미치는 影響

The Effect of Cellular Constituents Fractionated by High Speed Centrifigation on the Growth of Cancer

대한외과학회지 1967년 9권 8호 p.441 ~ 450
고재?,
소속 상세정보
고재? (  ) - 서울대학교 의과대학 외과학교실 서울대학교 의과대학 암연구소

Abstract


It has been well established that the mitochondria and microsome of the cell contain varying amounts of enzymes, especially those closely related with the tissue respiration and energy metabolism. Schneider, Hodgboom & Ross reported that the mitochondrias from the cancer tissue of the DAB rat liver are smaller in both size and number than those from the normal tissue. Shear, Syverton & Bittner indicated that, with high-speed centrifugal separation of the homogenized cancer tissue, the accelerant indexes are highest in the fresh accelerant, lower in both the mitochondrias and microsomes and are close to nil in the final supernatant. In an attempt to further the studies on the tissue growth promoting and inhibiting factors, the writer separated the mitochondria, microsome and the final supernatant and their alcohol-treated products from the human full-term placenta according to the method of high-speed centrifugation developed by Bittner et al.
In order to observe the effects on growth of tumor, these preparations were administered to the mice bearing the Ehrlich Cancer and N-F Sarcoma.
The following Results, were obtained.
1. The fractions of both the mitochodria and final supernatant inhibited growth of the tumors. The inhibition was more marked in the latter.
2. The microsomal fraction,on the other hand, promoted growth of the tumors.
3. When each fraction was treated with alcohol, the resultant supernatant promoted growth of the tumors, while the precipitate inhibited tumor growth.
4. The inhibiting effect of the alcohol-precipitated components of the mitochondria) and final supernatant
fractions was more pronounced than the promoting effect of the alcohol treated supernatant components,
the alcohol-precipitate component of the final supernatant fraction being most marked in the inhibiting
effect. In the microsomal fraction, the promoting effect of the alcohol-treated supernatant component was more marked 1.han the inhibiting effect of its alcohol precipitated component.

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