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The Metabolism of (4-^(14)C) Cholesterol on Photoperiodism in Solanum andigena

원자력학회지 1970년 2권 3호 p.179 ~ 183
Bae Moo, E.I. Mercer,
소속 상세정보
 ( Bae Moo ) - Korea Institute of Science and Technology
 ( E.I. Mercer ) - University of Wales Department of Biochemistry

Abstract

Solanum andigena의 광주성 주기중의 방사성 코래스테로올의 대사를 연구하였다. 대사산물로서 방사성 솔라닌과 그 가수분해물 솔라니딘을 분리 결정화하였으며, 코래스테로올은 이 식물체 내에서 형성되는 애스테르화된 형태로 분리하였다. 단일 및 장일식물간의 코래스테로올 대사에는 뚜렷한 차이는 찾아 볼 수 없으나, 괴경(塊莖)중의 스테로이드 알카로이드에 코래스테로올이 관여하고 있는 것으로 논의되었다.
(4140) Cholesterol was administered to the leaves of Solanum andigena during photoperiodic induction. Radioactive products converted from cholesterol were studied by thin-layer chromatography and gas-chromatography. The major products from cholesterol were shown to be esterified cholesterol in lipid and solanine in the aquoeus ethanolic phase in SD-and LD-leaves. The radioactive solanidine was isolated by chromatography and crystallized to constant specific activity. Short-day condition did not stimulate the conversion of cholesterol into solanine in the leaves, but both groups of leaves converted cholesterol into solanine at the about same rate. Incorporation of radioactivity int aqueous ethanolic phase of tubers and stolons, containing steriod glycoalkaloid, was very much higher than that into lipid phase, contrary to those in the leaves and the stems.

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