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赤血球 및 網狀赤血球의 乳酸脫水素 同位酵素에 關하여

Lactic Dehydrogenase Isoenzymes in Maturing Rabbit Reticulocytes

전남의대잡지 1966년 3권 1호 p.77 ~ 81
고재탁,
소속 상세정보
고재탁 (  ) - 전남대학교 의과대학 생화학교실

Abstract


Lactic dehydrogenase isoenzyme pattern in erythrocytes during maturation was studied in erythrocytes obtained from rabbits treated with phenylhydrazine, using a differential method for the heart type and muscle type isoenzymes with heat treatment at 60°C for 20 minutes.
Mature erythrocytes were found to contain predominantly the heart-type isoenzyme with barely detectable muscle-type isoenzyme. As the reticulocyte counts were increased the percentage of the muscle-type increased while that of the heart-type decreased proportionately. Total activity of the enzyme was also elevated in reticulocyte-rich hemolysate. Cessation of phenylhydrazine treatment brought about a return to normal erythrocyte pattern of the isoenzymes.

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