잠시만 기다려 주세요. 로딩중입니다.

組織 乳酸에 脫水素 同位酵素의 出生後 變動

Developmental Change of Tissue Lactic Dehydrogenase Isoenzymes in Neonatal Rabbits and Chickens

전남의대잡지 1966년 3권 1호 p.93 ~ 102
하기수,
소속 상세정보
하기수 (  ) - 전남대학교 의과대학 생화학교실

Abstract


Total lacticdehydrogenase (LDH) activity as. well as the relative distribution of heart and muscle type isoenzymes was assayed in tissues of neonatal rabbits and chickens for 35 days after birth. A differential method for the heart-and muscle-type isoenzymes with heat treatment at 60°C for 20 minutes was used.
1. Total LDH activity of the liver-and femoral muscle began to increase at the 6th day after birth and reached a maximum value of 2 to 3 fold increase at the 9th or 10th day with a rapid decrease. thereafter. Total activity of the heart also began to increase at the 6th day andcontlnued increasing thereafter, reaching a 2-fold increased value at the 35th day Erythrocytes did not show any variation in total activity during growth after birth.
2. LDH isoenzyme pattern of rabbit heart, skeletal muscle and erythrocyte did not show any significant change during growth after birth resuming already the characteristic adult tissue pattern from the onset of birth. Thus, more than 80% heart type LDH was found at birth in the heart and erythrocytes while muscle type LDH was seen in skeletal muscle in a predominant porion (more than 80%).
However, the liver underwent a drastic change in its composition of LDH isoenzymes during development after birth. During the early week after birth the predominant isoenzyme in liver was heart-type LDH. However, this heart-type LDH began to decrease around the 8th day after birth with a proportional increase of muscle-type LDH, and a complete reversal of the neonatal pattern occurred to resume the characteristic adult liver pattern after the 45th day of neonatal period.
3. In the tissues of neonatal chickens, no significant change after birth in the isoenzyme distribution was seen although the total activity increased in the heart, liver and skeletal muscle. The isoenzyme pattern as well as total activity, did not show any change in chicken erythrocytes.
4. The observed species-and tissue-specific changes in the isoenzyme distribution during development after birth are discussed in relation to the different maturity at birth of the animal and of different tissues in the same animal.

키워드

원문 및 링크아웃 정보

등재저널 정보