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家兎網狀赤血球의 Deoxyribonuclease의 部分精製와 그 性狀

Partial Purification and Properties of Deoxyribonuclease of Reticulocytes

전남의대잡지 1968년 5권 1호 p.23 ~ 34
윤재봉,
소속 상세정보
윤재봉 (  ) - 전남대학교 의과대학 생화학교실

Abstract


1. An acid deoxyribonuclease (RNase) has been, partially purified from rabbit reticulocytes, and its properties have been investigated.
2. The enzyme shows a sharp pH optimum at pH 5.0 and is I rapidly inactivated by beat over 60°C. The activity is inhibited by magnesium, manganese and sulfate-ions above 1.0 mM .
3. The enzyme is able to hydrolyze both native and heat-denatured DNA prepared from chicken erythrocytes, but fails to cleave calf thymus DNA. Yeast DNA can be attacked by this DNase.
4. Some inhibitors of protein nature is suggested to ble present in peripheral circulating erythrocytes and bone marrow nucleated erythrocytes of normal rabbits.
5. From the time activity relationship and analysis of reaction products in the enzymic digest, it is suggested that the endonucleolytic and edonucleolytic activities are associated with the enzyme.
6. The occurrence of the RNase only in reticulocytes is discussed in relation to DNA synthesis in nucleated erythrocytes of mammals.

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