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PEPSIN의 蛋白分解作用에 미치는 PAS의 影響에 關하여

Influence of PAS on the Proteolytic Action of Pepsin

중앙의학 1961년 1권 7호 p.759 ~ 762
강선제,
소속 상세정보
강선제 (  ) - 서울대학교 의과대학 외과학교실

Abstract


Experiments were carried out to observe the influence of PAS on the proteolytic action of pepsin.
To determine the effect of PAS,
1ml of pepsin solution(15mg/ml) was placed in a test tube at various pH and either 1ml, of water or PAS solution (15mg/ml) was added and the contents mixed by swirling after which 1ml of Human Serum Albumin was added.
Following an incubation period of 30minutes 10ml of 10 %Trichloracetic Acid was added to stop the reaction.
After removing supernatant, 2cc of 0 2N NaoH was added in test tube and 8cc of Biuret Reagent was added. Heparin solution (10mg/ml) is known as the inhibitant of pepsin activity and same test was done with heparin solution to compare the influence of PAS and heparin on the proteolytic action of pepsin.
The electrophotometric reading showed heparin inhibited the proteoly tic action at optimum pH of pepsin activity but PAS did not.

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