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非傳染性 phage lysate가 phage 血球凝集 反應에 미치는 影響에 關하여

Effect of non-infectious phage lysate on the phage heanagglutination

중앙의학 1967년 12권 3호 p.287 ~ 291
이현택,
소속 상세정보
이현택 (  ) - 전남대학교 의과대학 대학원

Abstract


The author has investigated the effect of non-infectious phage lysate on phage hemagglutination. For this experiment, the klebsiella phage, it´s host and goat red cells were used and obtained the following results.
By centrifugating of the phage suspension containing about 2x1011/ml of phage for 8 hours at about 17, 000 r.p.m., the phage was almost sedimented and about 7x103/ml of phage was still remained in the supernatent fluid.
The phage was not adsorbed to the sensitized red cells pretreated in non-infectious phage lysate.
When high-titering phage is added to the sensitized red cells pretreated in non-infectious phage lysate, the phage hemagglutination has slightly occurred in higher concentration of antiserum.
The activity of non-infectious phage lysate was neutralized by anti-phage serum and, by diluting, the activity of non-infectious phage lysate became rapidly lowered compared with that of phage under the same grade of dilution.

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