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骨折 및 Cortisone, DOCA 投與가 骨組織內 glutamic acid 代謝에 미치는 影響

Effect of fracture and administration of cortisone and DOCA on the metabolism of glutamic acid in femur of mouse.

중앙의학 1967년 13권 3호 p.298 ~ 306
劉道順, 李震淳,
소속 상세정보
劉道順 (  ) - 서울대학교 醫科大學 生化學敎室
李震淳 (  ) - 서울대학교 醫科大學 生化學敎室

Abstract


Mice were subjected to investigate the changes in metabolism of glutamic acid´-C14 following bone fracture on left femur and administration of cortisone and DOCA in vivo and in vitro.
In vivo experiment 1. 5mg of cortisone acetate and 0. 3m g of DOCA were infected to each mouse hypodermally in 3 days and closed bone fracture was performed just after the last injection. Two days after bone fracture mouse was sacrificed by decapitation and both femur were isolated to pulverize in mortar and homogenize with incubation media containing glutamic acid-C14.
In the case of in vitro experiment, two days after bone fracture, mouse was sacrificed and both femurs were taken out in order to incubate with cortisone and DOCA for 24 hours. After incubation with steroids the bone was pulverized and homogenized to incubate with incubation media containing glutamic acid-C14.
After the incubation determinations of CO2 production rate and specific activity of respiratory CO2, amount of protein, specific activity of protein and specific activity of non- protein filtrate, were performed by means of chemical balancing and radioactivity counting.
The result of this experiment was as follow.
1. The production of CO2 was increased by bone specific activity of CO2 was decreased.
2. The content of protein and specific activity of protein were increased by bone fracture, while decreased by cortisone.
3. The radioactivity of non-protein filtrate was decreased by bone fracture and was increased by cortisone
4. DOCA did not bring any significant change in every aspect and the effect of cortisone in vivo did not differ from in vitro.

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