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各種體液의 組織成長促進性 및 抑制性物質에 關한 影響

Tissue Growth Promoting and Inhibiting Substances in Various Body Fluids

중앙의학 1967년 13권 5호 p.471 ~ 478
이준기,
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이준기 (  ) - 서울대학교 의과대학 외과학교실

Abstract


Many workers have reported that substances either inhibiting or promoting tumor and normal tissue growth can be obtained from various organs and tumors.
The writer extracted both the promoting and inhibiting factors separately from -various body fluids, namely ascities, pus and bile, and attempted to investigate their effect on cancer of mice.
Materials and Methods
The cancer strain used in the present work was the Ehrlich ascites cancer. Tumor transplantation was carried out after the Hwang´s method and extraction of tissue factors after the Lee´s method. Tissue factors were prepared from various body fluids in the following manner;
Various body fluids (ascites, pus and bile) were centrifuged in Model P.R.I. International centrifuge at 15, 000 r. p. m. Ethly alcohol was added to the supernatant and the resultant alcohol soluble supernatant (HAS) and insoluble sediment (HAP) were fractionated and evaporated to complete dryness. The residues from both fractions were dissolved in the distilled water in order to make a 5% solution for injection.
After tumor was transplanted to the animals, the injection of tissue factor was carried out in the following manner.
A single does of either 0.1m1 or 0.2m1 of the tissue factor solution was injected into the experimental mice once daily.
1) The average of the two diameters of each tumor mass was then measured and compared with those of the control group.
2) On the 18th day following tumor transplantation, the animals were sacrificed and the tumors were removed and weighed.
Results
Ethyl alcohol was added to the supernatant obtained by centrifusing the various
body fluids;
1) H.A. P. generally inhibits the growth of Ehrlich cancer.
2) H. A. S. promotes the growth of Ehrlich cancer. These inhibiting and promoting
effects were more marked with the increase in the dosage of injection.
3) It was disclosed that both factors which promote or inhibied the growth of cancer
tissue exist in the various body fluids.

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