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病原性好鹽菌의 細菌學的性狀 및 病原性에 關한 硏究

STUDIES ON THE BACTERIOLOGICAL CHARACTERISTICS AND PATHOGENICITY OF PATHOGENIC HALOPHILIC ORGANISMS

중앙의학 1968년 14권 6호 p.535 ~ 542
김영권,
소속 상세정보
김영권 (  ) - 경북대학교 의과대학 세균학교실

Abstract


The bacteriological characteristics and the pathogenicity of enteropathogenic halophilic vibrios isolated from sea-fishes and shell-fishes collected in the port of Pohang, Kyungpook during July and August of 1966 and 1967 were studied, and the following results were obtained.
1. Vibrios were classified into three species, i.e. Vibrio parahaemolitycus, V. alginolyticus, and V. anguillarum. The criteria of classification were based on the growth on nutrient agar containing 7 and 10 per cent of sodium chloride, Voges-Proskauer reaction, swarming growth on nutrient agar, and fermentation of sucrose and cellobiose.
2. The biological characteristics of vibrios were, in general, uniform among strains in each. species, and three species of vibrios showed their typical characteristics in each species..
3. Most of test organisms can grow well in trypticase soy broth of pH 4.5-5.0, and there were a few strains which can grow in media of pH 6. 0
4. Most strains were resistant to penicillin, methicillin, and cloxacillin, and grow well in the presence of 200-100u or mcg/ml of these antibiotics. The resistance to streptomycin and erythromycin was moderate and the minimum inhibitory concentration (M.I.C.) was 50 mcg/ml or more in most strains. However, the sensitivity to chloramphenicol, aureomycin, terramycin, and tetracyline was very high with the M.I.C. of 1.6mcg/ml, and there were some strains with the M.I.C. of 3.1-6. 2mcg/ml including a few strains with the M.I.C. of 6.2mcg/ml.
5. One-half to one-fourth ml of broth culture containing 3.8-8.3X108/ml of viable cells were injected into the peritoneal cavity of mouse, and the death was observed.

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