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放射性硫黃(S^(35))을 利用한 齒牙硬組織形成時의 硫化鹽에 關한 實驗的硏究

Autoradiographical Observations of Radioactive Sulfur (S^(35)) on the Mineralization of Dental Hard Tissues in Growing Rats

최신의학 1966년 9권 12호 p.77 ~ 83
박광진,
소속 상세정보
박광진 (  ) - 서울대학교 치과대학 예방치과학교실

Abstract


Radioactive Sulfur were peritoneal and subcutaneously injected into the suckling ratg* and traced in dental hard tissuse at various time intervals with macro-, and microradioautography in accordance with the metachromic staining with toluidin blue.
Thirty suckling rats were divided into two groups, 15 in each, with the ages of 7 and 14 days old and were peritoneally injected with a single dose of 20 pc S35-sulfate per gram of body weight just 24 hours before the sacrifice in first experiments and, in second experiments, rats of 4, 6, 8, and 10 days of age, 5 animals in each, were injected subcutaneously with a single dose of 20 pc per gram of body weight of S35-separated isotope, obtained as weak H2SO4. These animals were sacrificed at intervals of one and two hours and one, two, four and eight days thereafter.
Following were the results:
1) Radiosuifate injected peritoneally to suckling rats were detected autoradiographically in the apical , portion where ameloblasts and dental hard tissue formation were,selective initiated and near the layers of cdontoblasts and ameloblasts were also same.
2) In the pulp, a faint blackening appeared in the vicinity of the blood vessels and the layers of matured enamel does not revealed the distribution of radioactivity. Periodontal membrane showed the radioactivity similar to that of dental pulp.
3) Radiosulfate injected, peritoneally formed a complex with mucopolysaccharide, which appeared to play important role in the ,development and formation of dental hard tissue. The sulfated complex was dissolved out by 4 nitric acid during the decalcification process.
4) The distribution of radioactive sulfur was more aboundant in seven days of age than rats of 14 days of age and more in grinding section than decalcified samples.
5) Radioactive sulfur seems to be synthesized into a sulfuric compound which appears in enamel, matrix and disappears progressively with the start of mineralization.

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