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細胞成長促進因子의 特性에 關한 硏究

Properties of Cell Growth Promoting Tissue Factor

최신의학 1968년 11권 5호 p.75 ~ 82
홍순원,
소속 상세정보
홍순원 (  ) - 서울대학교 의과대학 외과학교실 및 암연구소

Abstract


As animal cancers, Ehrlich ascites cancer was used and tumor transplantation was performed into experir2´ !
mental mice.
Tissue growth promoting factors was extracted from liver, spleen, kidney, brain and muscle of the Hybrid rats. Liver of dog, rabbit and mouse were used as comparison factors.
1. The tissue promting factors(extracted as Chin´s Nlethod)from liver was prepared in both low and high concentration and injected separately into the tumor transplanted mice and tumor growth compared both and control group.
2. The tissue factors were divided into the filtrable and dialyzable and the non-filtrable part and similar experiment carried out.
3. Fraction 1, 11 and III were obtained by ultracentrifugation.
4. The promoting tissue factor was treated by trypsin, HCl, ethanol and methanol.
5. The extract was treated with NaOH, ether and Trichloracetic acid(TCA).
6. The extract was heated at 40 C.,56´ C. and 100 C. and also frozen at-20C. and OC.
7. Extracts were obtained from spleen, kideny, brain and skeletal muscle as well as from liver of rats and injected into experimental mice.
8. Extracts were obtaineed from liver of dog, rabbit and mouse. as well as rat and injected into exper ´mental mice.
9. Extract obtained from liver of rats was injected together with Ehrlich ascites on the back of mouse and compared different site injection(abdomen, intraperitoneally).
Results
The factor promotes tumor growth in mice at high concentration, but inhibits tumor growth at very high concentration.
2. The factor is unfiltrable and undialysable.
3. The factor is included mostly in the microsome(Fraction )i ) and mitochondria(Fraction I) of the ultracentrifugation.
4. Trypsin, Hcl, ethanol and methanol have no effect on the promoting factor, while TCA, NaoH and ether neutralize the action of the promoting factor.
5. The factor is heat-labile and cold-stable.
6. The factor does possess species specificity.
7. The promoting action of the factor is most pronounced in the extract from liver.
8. The promoting action of the factor is most prononnced when it is injected together with tnmor cell sus pension.

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