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Properties of Gas Diffusion in Excised Cornea of Dog and Rabbit.
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KMID : 0378019680110100071
Abstract
Diffusion properly of the cornea to CO2 has studied in present report. Fresh cornea was excised from dog or rabbit and was tightly ligated to, the open end of a small glass capsule. The space of bigger glass box in which glass; capsule with cornea sample is contained was filled with pure CO2 under positive pressure of 3 mmHg. The other end¢¥ of glass capsule was connected to two 10 nil syringes by two narrow polyethylen tubes and the total gas space of the capsule, tubes and syringes was arranged as to be 9 ml filled with room air.,
The volume of diffused COZ into ¢¥the capsule from outer space was measured by analysis of the CO2 composition in the-capsule space. The diffusion rate of CO2 was measured at each 5th minute for 30 minutes of experiment.
Results obtained are summarized as follow:. "
1. In 9¢¥samples of dog cornea, CO2 diffusion rate was 5.4 XW-3 ml/min/cmz in average of 30 minutes duration and in first ¢¥5 minutes, -the diffusion rate was 7.87 X10-3 ml/min/emz.
In 4 samples of rabbit cornea, ~COZ diffusion rate was 7.54 X10^(-3 )ml/min/cmz in average of 30 minutes duration and was 10.5 X 10-3 ml/min/=2 in first 5 minutes.. 7
2., Diffusion coefficient of CO2 in dog cornea in first 5 minutes is calculated as 10.55 X10-4 cm2/min and permeation . coefficient of CO2 in dog cornea is calculated as 5.89 X 10-4 cui2/min/atm.
Diffusion coefficient¢¥ and permeation coefficient of 02 in.dog cornea. is driven from those of¢¥ .0O2. in dog
cornea and is estimated as 10.88 X 10-4 cm.2/min and 2.56 X 10^(-3)cm2/min/atm. respectively
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