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節足動物媒介性 바이러스의 Plaque 形成에 關한 硏究

The Enhancement of Plaque Formation in Tissue Culture (VERO-cell) by Arboviruses

최신의학 1969년 12권 3호 p.51 ~ 57
李淵台, 朴容祥,
소속 상세정보
李淵台 (  ) - 가톨릭大學 醫學部 微生物學敎室
朴容祥 (  ) - 가톨릭大學 醫學部 微生物學敎室

Abstract


A number of. arboviruses, such as group A (SF, Chik, WEE) and group B (D-2, RSSE, WN) multiply in mammalian cells but some fail to proceduce plaques -in them when grown under an agar overlay.
The growth. of the plaque by :SF, Chik, WM, Dengue type 2, RSSE, WN viruses were sonie inhibited :in 5% &im milk in agar but were ,favourably .;influenced by addition -of DEAF-dextran in optimal .concentration, 50 to :100: .14g/nil to . agar.. Addition of rprotamine sulfate in optimal concentration, 50 to 100 pg/ml, also increased the plaque formation by.SF,, ck, WEE and WN viruses tested.
The addition of these chemical reagents in optimal concentrations do not inhibit plaque sizes and number, those plaques .by some viruses bear ;polymorphic .ppearances.

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