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Effects of canine mesenchymal stem cells-derived exosomes in a mouse model of canine mammary tumor

예방수의학회지 2020년 44권 4호 p.147 ~ 155
정소연, 이세아, 구나연, 이지현, 이윤희, 현방훈,
소속 상세정보
정소연 ( Jeong So-Yeon ) - Animal and Plant Quarantine Agency
이세아 ( Lee Se-A ) - Animal and Plant Quarantine Agency
구나연 ( Gu Na-Yeon ) - Animal and Plant Quarantine Agency
이지현 ( Lee Jienny ) - Animal and Plant Quarantine Agency
이윤희 ( Lee Yoon-Hee ) - Animal and Plant Quarantine Agency
현방훈 ( Hyun Bang-Hun ) - Animal and Plant Quarantine Agency

Abstract


Canine mammary tumors account for ~30% of all tumors in the female dogs and approximately 50% of the tumors are malignant. Exosomes have been the focus of great interest, as they appear to be involved in numerous important cellular processes. In this study, we examined the anti-tumor effects of canine mesenchymal stem cells-derived exosomes (MSC-exosomes) in an experimental murine mammary tumor model using canine mammary carcinoma cells, REM134. The MSC-exosomes were injected tumor site and tail vein of REM134 xenografted mice. We found that tumor size of the MSC-exosomes-treated group decreased compared to those of the only tumor group in REM134-driven tumorigenic mouse model. In addition, the MSC-exosomes-treated tumor group showed meaningfully reduced expression levels of the MMP-3, IL-1β, IL-6, and TNF-α compared to those in the tumor group. Specifically, we confirmed that the expression level of the CD133, potent cancer stem cell (CSC) markers, decreased in the MSC-exosomes-treated tumor group compared to the tumor group. This study suggests that the MSC-exosomes exhibited anti-tumor effects through downregulating CSC-related markers in the canine mammary tumor murine model. Further study is needed in the future, and we are conducting research on the detailed anti-tumor mechanism of the MSC-exosomes.

키워드

Canine mammary tumor; cancer stem cells; mesenchymal stem cells; exosomes; anti-tumor effect

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