잠시만 기다려 주세요. 로딩중입니다.

Comparative assessment of the biological toxicity of scyphozoan jellyfish species (Nemopilema nomurai, Cyanea nozakii, Aurellia aurita and Rhopilema esculentum) venoms

예방수의학회지 2020년 44권 4호 p.179 ~ 185
양혜련, 이예현, 성종배, 최석문, Hwang Du-Hyeon, 김의경, 강창근,
소속 상세정보
양혜련 ( Yang Hye-Ryeon ) - Gyeongsang National University College of Veterinary Medicine
이예현 ( Lee Ye-Hyeon ) - Gyeongsang National University College of Veterinary Medicine
성종배 ( Seong Jong-Bae ) - Gyeongsang National University College of Veterinary Medicine
최석문 ( Choi Suk-Moon ) - Gyeongsang National University College of Veterinary Medicine
 ( Hwang Du-Hyeon ) - Gyeongsang National University College of Veterinary Medicine
김의경 ( Kim Eui-Kyung ) - Gyeongsang National University College of Veterinary Medicine
강창근 ( Kang Chang-Keun ) - Gyeongsang National University College of Veterinary Medicine

Abstract


Jellyfish envenomation is a world-wide health problem, which often seriously affect the fishery and bathing activities. To date, few individual jellyfish venoms proteins have been thoroughly characterized yet. In this work, four species of scyphozoan jellyfish (Nemopilema nomurai, Cyanea nozakii, Aurellia aurita and Rhopilema esculentum) are compared according to their, cytotoxicity, hemolytic potency, brine shrimp toxicity and protein components. Jellyfish venoms showed higher cytotoxicity in H9C2 heart myoblast than in C2C12 skeletal myoblast, with the exception of C. nozakii venom. This result suggests that the selective cytotoxic effects may be possibly related to their in vivo effects of cardiac tissue dysfunction. On the other hand, hemolytic activity could be also observed from all tested jellyfish venoms. N. nomurai jellyfish venom displaying the greatest hemolytic activity. As an alternative method of evaluating the toxicities of jellyfish venoms, the toxicity on brine shrimp was examined with the four jellyfish venoms. From this, the venom of N. nomurai showed higher toxicity against brine shrimp than the other jellyfish venoms, which is consistent with the results of cytotoxicity assay as well as hemolysis assay of the present study. SDS-PAGE analysis of four jellyfish venoms showed the similar pattern with molecular weight of around 40 kDa, and appeared to be the major protein components. These results provided that N. nomurai jellyfish venom was potently toxic than other scyphozoan jellyfish venoms and may explain to some extent the deleterious effects associated with human envenoming.

키워드

Scyphozoan jellyfish; venom; cytotoxicity; hemolysis; brine shrimp toxicity

원문 및 링크아웃 정보

등재저널 정보