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Ovarian transcriptome profiles associated with sexual maturation in Pacific abalone (Haliotis discus hannai)

Genes & Genomics 2020년 42권 10호 p.1179 ~ 1188
김미애, 김태하, 이소라, 남보혜, 이정식, 장원희, 손영창,
소속 상세정보
김미애 ( Kim Mi-Ae ) - Gangneung-Wonju National University Department of Marine Molecular Bioscience
김태하 ( Kim Tae-Ha ) - Gangneung-Wonju National University Department of Marine Molecular Bioscience
이소라 ( Lee So-Ra ) - Dongguk University Department of Life Science
남보혜 ( Nam Bo-Hye ) - National Institute of Fisheries Science Biotechnology Research Division
이정식 ( Lee Jung-Sick ) - Chonnam National University Department of Aqualife Medicine
장원희 ( Jang Won-Hee ) - Dongguk University Department of Life Science
손영창 ( Sohn Young-Chang ) - Gangneung-Wonju National University Department of Marine Molecular Bioscience

Abstract


Background: There is now abundant information on genes involved in molluscan oogenesis and their associations with ovarian development. However, few studies have investigated the ovarian transcriptome of Pacific abalone (Haliotis discus hannai).

Objective: The objective of this study was to identify genes related to ovarian development and maturation in Pacific abalone utilizing RNA-sequencing (RNA-seq) and to verify the genes most relevant to different stages of maturation.

Methods: RNA samples from the ovarian tissues of sexually immature and mature abalone were used to construct cDNA libraries, which were paired-end sequenced on an Illumina HiSeq 2500 platform. Reads from individual samples (unigenes) were aligned to reference transcriptome databases for identification of differentially expressed genes (DEGs) between immature and mature ovarian libraries. Reverse transcription-quantitative polymerase chain reaction was used to verify the RNA-seq data.

Results: A total of 8779 unigenes were obtained from the ovaries of immature and mature abalone, with a total length of 3323,279 bp and an average length of 379 bp per gene. Gene ontology analysis assigned 5860 unigenes to biological processes, 855 to cellular components, and 1352 to molecular functions. Overall, 470 DEGs were identified, including 213 and 257 genes down-regulated and up-regulated in mature abalone, respectively. Among these, 13 relevant transcripts, including VTG1 and FZD7, were significantly highly expressed in the ovaries of mature abalone (p??2).

Conclusion: This H. discus hannai ovary transcriptome provides molecular targets to better understand ovarian development, oogenesis, and sexual maturation, and to enhance Pacific abalone production.

키워드

Differentially expressed genes; Haliotis discus hannai; Ovary; Pacific abalone; RNA sequencing

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