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Expression of TAS2R14 in the intestinal endocrine cells of non-human primates

Genes & Genomics 2021년 43권 3호 p.259 ~ 267
Hayashi Misa, Inaba Akihiko, Hakukawa Miho, Iwatsuki Ken, Imai Hiroo, Masuda Katsuyoshi,
소속 상세정보
 ( Hayashi Misa ) - Kyoto University Primate Research Institute Department of Cellular and Molecular Biology
 ( Inaba Akihiko ) - Kyoto University Primate Research Institute Department of Cellular and Molecular Biology
 ( Hakukawa Miho ) - Kyoto University Primate Research Institute Department of Cellular and Molecular Biology
 ( Iwatsuki Ken ) - Tokyo University of Agriculture Faculty of Applied Bioscience Department of Nutritional Science and Food Safety
 ( Imai Hiroo ) - Kyoto University Primate Research Institute Department of Cellular and Molecular Biology
 ( Masuda Katsuyoshi ) - Kyoto University Graduate School of Medicine Structural Bioscience for Taste Molecular Recognition

Abstract


Background: Recent studies have demonstrated that genes related to bitter taste receptors (TAS2Rs) on various chromosomes are expressed in extra-oral organs of various animals. The bitter taste receptor TAS2R14 is conserved among primate species and shows broad ligand sensitivity. Mice have a number of orthologues to primate TAS2R14 located in tandem on chromosome 16; however, their expression patterns are not unique.

Objective: We characterized the expression of TAS2R14 in various cell types in the intestines of the rhesus macaque and evaluated its role in hormone production in the gut.

Methods: TAS2R14 expression was examined in the intestines of rhesus macaques, a common non-human primate model, by RT-qPCR and immunohistochemical staining.

Results: Mean expression levels of TAS2R14 in the duodenum, ileum, and colon were similar to each other and were lower than those in circumvallate papillae. An immunohistochemical analysis revealed TAS2R14 immunoreactivity in enteroendocrine cells positive for cholecystokinin, serotonin, and the G protein GNAT3.

Conclusion: These results suggest that primate TAS2R14 is broadly expressed in the intestine, mainly in enteroendocrine cells, and promotes gut hormone secretion in response to bitter stimuli.

키워드

TAS2R14; Enteroendocrine; CCK; 5-HT; Non-human primates

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