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Morphological and Molecular Diagnosis of Necator americanus and Ancylostoma ceylanicum Recovered from Villagers in Northern Cambodia

Korean Journal of Parasitology 2020년 58권 6호 p.619 ~ 625
장태희, 정봉관, 손운목, 홍수지, Shin Hye-Joo, Ryoo Seung-Wan, 이정규, Lee Keon-Hoon, Khieu Virak, Huy Rekol, 채종일,
소속 상세정보
장태희 ( Chang Tae-Hee ) - Korea Association of Health Promotion Institute of Parasitic Diseases
정봉관 ( Jung Bong-Kwang ) - Korea Association of Health Promotion Institute of Parasitic Diseases
손운목 ( Sohn Woon-Mok ) - Gyeongsang National University School of Medicine Department of Parasitology and Tropical Medicine
홍수지 ( Hong Soo-Ji ) - Korea Association of Health Promotion Institute of Parasitic Diseases
 ( Shin Hye-Joo ) - Korea Association of Health Promotion Institute of Parasitic Diseases
 ( Ryoo Seung-Wan ) - Korea Association of Health Promotion Institute of Parasitic Diseases
이정규 ( Lee Jeong-Gyu ) - Korea Association of Health Promotion Institute of Parasitic Diseases
 ( Lee Keon-Hoon ) - Korea Association of Health Promotion Institute of Parasitic Diseases
 ( Khieu Virak ) - Ministry of Health National Center for Parasitology, Entomology and Malaria Control
 ( Huy Rekol ) - Ministry of Health National Center for Parasitology, Entomology and Malaria Control
채종일 ( Chai Jong-Yil ) - Korea Association of Health Promotion Institute of Parasitic Diseases

Abstract


Human hookworm infections caused by adult Ancylostoma spp. and Necator americanus are one of the most important tropical diseases. We performed a survey of intestinal helminths using the Kato-Katz fecal examination technique targeting 1,156 villagers residing in 2 northern provinces (Preah Vihear and Stung Treng) of Cambodia in 2018. The results revealed a high overall egg positive rate of intestinal helminths (61.9%), and the egg positive rate of hookworms was 11.6%. Nine of the hookworm egg positive cases in Preah Vihear Province were treated with 5-10 mg/kg pyrantel pamoate followed by purging with magnesium salts, and a total of 65 adult hookworms were expelled in diarrheic stools. The adult hookworms were analyzed morphologically and molecularly to confirm the species. The morphologies of the buccal cavity and dorsal rays on the costa were observed with a light microscope, and the nucleotide sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene were analyzed. The majority of the hookworm adults (90.7%) were N. americanus, whereas the remaining 9.3% were Ancylostoma ceylanicum, a rare hookworm species infecting humans. The results revealed a high prevalence of hookworm infections among people in a northern part of Cambodia, suggesting the necessity of a sustained survey combined with control measures against hookworm infections.

키워드

Necator americanus; Ancylostoma ceylanicum; fecal examination; morphological diagnosis; molecular diagnosis; cox1; Cambodia

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