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Morphological and Molecular Identification of Spirometra Tapeworms (Cestoda: Diphyllobothriidae) from Carnivorous Mammals in the Serengeti and Selous Ecosystems of Tanzania

Korean Journal of Parasitology 2020년 58권 6호 p.653 ~ 660
Ndosi Barakaeli Abdieli, 박한솔, 이동민, 최성준, 강예슬, Nath Tilak Chandra, Bia Mohammed Mebarek, Eamudomkarn Chatanun, 전형규, 엄기선,
소속 상세정보
 ( Ndosi Barakaeli Abdieli ) - Chungbuk National University School of Medicine Department of Parasitology
박한솔 ( Park Han-Sol ) - Chungbuk National University School of Medicine Department of Parasitology
이동민 ( Lee Dong-Min ) - Chungbuk National University School of Medicine Department of Parasitology
최성준 ( Choe Seong-Jun ) - Chungbuk National University School of Medicine Department of Parasitology
강예슬 ( Kang Ye-Seul ) - Chungbuk National University School of Medicine Department of Parasitology
 ( Nath Tilak Chandra ) - Chungbuk National University School of Medicine Department of Parasitology
 ( Bia Mohammed Mebarek ) - Chungbuk National University School of Medicine Department of Parasitology
 ( Eamudomkarn Chatanun ) - Khon Kaen University Faculty of Medicine Department of Parasitology
전형규 ( Jeon Hyeong-Kyu ) - Chungbuk National University School of Medicine Department of Parasitology
엄기선 ( Eom Kee-Seon S. ) - Chungbuk National University School of Medicine Department of Parasitology

Abstract


Spirometra tapeworms (Cestoda: Diphyllobothriidae) collected from carnivorous mammals in Tanzania were identified by the DNA sequence analysis of the mitochondrial cytochrome c oxidase subunit 1 (cox1) and internal transcribed spacer 1 (ITS1), and by morphological characteristics. A total of 15 adult worms were collected from stool samples and carcasses of Panthera leo, Panthera pardus, and Crocuta crocuta in the Serengeti and Selous ecosystems of Tanzania. Three Spirometra species: S. theileri, S. ranarum and S. erinaceieuropaei were identified based on morphological features. Partial cox1 sequences (400 bp) of 10 specimens were revealed. Eight specimens showed 99.5% similarity with Spirometra theileri (MK955901), 1 specimen showed 99.5% similarity with the Korean S. erinaceieuropaei and 1 specimen had 99.5% similarity with Myanmar S. ranarum. Sequence homology estimates for the ITS1 region of S. theileri were 89.8% with S. erinaceieuropaei, 82.5% with S. decipiens, and 78.3% with S. ranarum; and 94.4% homology was observed between S. decipiens and S. ranarum. Phylogenetic analyses were performed with 4 species of Spirometra and 2 species of Dibothriocephalus (=Diphyllobothrium). By both ML and BI methods, cox1 and ITS1 gave well supported, congruent trees topology of S. erinaceieuropaei and S. theileri with S. decipiens and S. ranarum forming a clade. The Dibothriocephalus species were sisters of each other and collectively forming successive outgroups. Our findings confirmed that 3 Spirometra species (S. theileri, S. ranarum, and S. erinaceieuropaei) are distributed in the Serengeti and Selous ecosystems of Tanzania.

키워드

Spirometra erinaceieuropaei; Spirometra ranarum; Spirometra theileri; cox1; ITS1

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