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Functional Identification of a Nuclear Localization Signal of MYB2 Protein in Giardia lamblia

Korean Journal of Parasitology 2020년 58권 6호 p.675 ~ 679
김주리, 신미영, 박순정,
소속 상세정보
김주리 ( Kim Ju-Ri ) - Yonsei University College of Medicine Department of Environmental Medical Biology
신미영 ( Shin Mee-Young ) - Yonsei University College of Medicine Department of Environmental Medical Biology
박순정 ( Park Soon-Jung ) - Yonsei University College of Medicine Department of Environmental Medical Biology

Abstract


MYB2 protein was identified as a transcription factor that showed encystation-induced expression in Giardia lamblia. Although nuclear import is essential for the functioning of a transcription factor, an evident nuclear localization signal (NLS) of G. lamblia MYB2 (GlMYB2) has not been defined. Based on putative GlMYB2 NLSs predicted by 2 programs, a series of plasmids expressing hemagglutinin (HA)-tagged GlMYB2 from the promoter of G. lamblia glutamate dehydrogenase were constructed and transfected into Giardia trophozoites. Immunofluorescence assays using anti-HA antibodies indicated that GlMYB2 amino acid sequence #507?#530 was required for the nuclear localization of GlMYB2, and this sequence was named as NLSGlMYB2. We further verified this finding by demonstrating the nuclear location of a protein obtained by the fusion of NLSGlMYB2 and G. lamblia glyceraldehyde 3-phosphate dehydrogenase, a non-nuclear protein. Our data on GlMYB2 will expand our understanding on NLSs functioning in G. lamblia.

키워드

Giardia lamblia; nuclear localization signal; GlMYB2

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