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Differentially Expressed Gene Profile of Acanthamoeba castellanii Induced by an Endosymbiont Legionella pneumophila

Korean Journal of Parasitology 2021년 59권 1호 p.67 ~ 75
문은경, 박소민, 추기백, Quan Fu-Shi, 공현희,
소속 상세정보
문은경 ( Moon Eun-Kyung ) - Kyung Hee University School of Medicine Department of Medical Zoology
박소민 ( Park So-Min ) - Kyung Hee University Graduate School Department of Biomedical Science
추기백 ( Chu Ki-Back ) - Kyung Hee University Graduate School Department of Biomedical Science
 ( Quan Fu-Shi ) - Kyung Hee University School of Medicine Department of Medical Zoology
공현희 ( Kong Hyun-Hee ) - Dong-A University College of Medicine Department of Parasitology

Abstract


Legionella pneumophila is an opportunistic pathogen that survives and proliferates within protists such as Acanthamoeba spp. in environment. However, intracellular pathogenic endosymbiosis and its implications within Acanthamoeba spp. remain poorly understood. In this study, RNA sequencing analysis was used to investigate transcriptional changes in A. castellanii in response to L. pneumophila infection. Based on RNA sequencing data, we identified 1,211 upregulated genes and 1,131 downregulated genes in A. castellanii infected with L. pneumophila for 12 hr. After 24 hr, 1,321 upregulated genes and 1,379 downregulated genes were identified. Gene ontology (GO) analysis revealed that L. pneumophila endosymbiosis enhanced hydrolase activity, catalytic activity, and DNA binding while reducing oxidoreductase activity in the molecular function (MF) domain. In particular, multiple genes associated with the GO term ‘integral component of membrane’ were downregulated during endosymbiosis. The endosymbiont also induced differential expression of various methyltransferases and acetyltransferases in A. castellanii. Findings herein are may significantly contribute to understanding endosymbiosis of L. pneumophila within A. castellanii.

키워드

Acanthamoeba; Legionella; endosymbiosis; differential gene expression

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