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Anti-Inflammatory Activity of Antimicrobial Peptide Periplanetasin-5 Derived from the Cockroach Periplaneta americana

Journal of Microbiology and Biotechnology 2020년 30권 9호 p.1282 ~ 1289
김인우, 이준하, 서민철, 이화정, 백민희, 김미애, 신용표, 김성현, 김익수, 황재삼,
소속 상세정보
김인우 ( Kim In-Woo ) - Rural Development Administration National Institute of Agricultural Sciences Department of Agricultural Biology
이준하 ( Lee Joon-Ha ) - Rural Development Administration National Institute of Agricultural Sciences Department of Agricultural Biology
서민철 ( Seo Min-Chul ) - Rural Development Administration National Institute of Agricultural Sciences Department of Agricultural Biology
이화정 ( Lee Hwa-Jeong ) - Rural Development Administration National Institute of Agricultural Sciences Department of Agricultural Biology
백민희 ( Baek Min-Hee ) - Rural Development Administration National Institute of Agricultural Sciences Department of Agricultural Biology
김미애 ( Kim Mi-Ae ) - Rural Development Administration National Institute of Agricultural Sciences Department of Agricultural Biology
신용표 ( Shin Yong-Pyo ) - Rural Development Administration National Institute of Agricultural Sciences Department of Agricultural Biology
김성현 ( Kim Sung-Hyun ) - Rural Development Administration National Institute of Agricultural Sciences Department of Agricultural Biology
김익수 ( Kim Ik-Soo ) - Chonnam National University College of Agriculture and Life Sciences
황재삼 ( Hwang Jae-Sam ) - Rural Development Administration National Institute of Agricultural Sciences Department of Agricultural Biology

Abstract


Previously, we performed an in silico analysis of the Periplaneta americana transcriptome. Antimicrobial peptide candidates were selected using an in silico antimicrobial peptide prediction method. It was found that periplanetasin-5 had antimicrobial activity against yeast and grampositive and gram-negative bacteria. In the present study, we demonstrated the anti-inflammatory activities of periplanetasin-5 in mouse macrophage Raw264.7 cells. No cytotoxicity was observed at 60 μg/ml periplanetasin-5, and treatment decreased nitric oxide production in Raw264.7 cells exposed to lipopolysaccharide (LPS). In addition, quantitative RT-PCR and enzyme-linked immunosorbent assay revealed that periplanetasin-5 reduced cytokine (tumor necrosis factor-α, interleukin-6) expression levels in the Raw264.7 cells. Periplanetasin-5 controlled inflammation by inhibiting phosphorylation of MAPKs, an inflammatory signaling element, and reducing the degradation of IκB. Through LAL assay, LPS toxicity was found to decrease in a periplanetasin-5 dose-dependent manner. Collectively, these data showed that periplanetasin-5 had antiinflammatory activities, exemplified in LPS-exposed Raw264.7 cells. Thus, we have provided a potentially useful antibacterial peptide candidate with anti-inflammatory activities.

키워드

Periplanetasin-5; antimicrobial peptide; anti-inflammatory activity; cockroach; Periplaneta americana

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