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Serum Levels and Glycosylation Changes of Alpha-1-Acid Glycoprotein According to Severity of Breast Cancer in Korean Women

Journal of Microbiology and Biotechnology 2020년 30권 9호 p.1297 ~ 1304
최재웅, 정기호, You Ji-Won, 이준우, 문병인, 김형진, 김홍진,
소속 상세정보
최재웅 ( Choi Jae-Woong ) - Chung-Ang University College of Pharmacy
정기호 ( Jeong Ki-Ho ) - Chung-Ang University College of Pharmacy
 ( You Ji-Won ) - Chung-Ang University College of Pharmacy
이준우 ( Lee Jun-Woo ) - Ewha Womans University Cancer Center for Women Breast and Thyroid Cancer Center
문병인 ( Moon Byung-In ) - Ewha Womans University Cancer Center for Women Breast and Thyroid Cancer Center
김형진 ( Kim Hyoung-Jin ) - Chung-Ang University College of Pharmacy
김홍진 ( Kim Hong-Jin ) - Chung-Ang University College of Pharmacy

Abstract


Elevated serum levels of alpha-1-acid glycoprotein (AGP) are known to be associated with several types of cancer. In addition, some reports have indicated that changes in glycosylation of AGP are associated with cancer progression. However, changes in AGP levels of serum and changes in glycosylation of AGPs in breast cancer have not been specifically studied. In the present study, serum AGP levels in benign (BN) cancer and breast cancer stage I (BC I), BC IIA, BC IIB, and BC III in Korean women were measured using an enzyme-linked immunosorbent assay (ELISA). AGP was purified from individual sera by hot phenol extraction and then subjected to AGP glycosylation analysis. Three types of AGP glycosylation (fucosylation, high-mannose-type and sialylation) were detected using enzyme-linked lectin assays (ELLAs). Serum AGP levels were higher in BC I, BC IIA, BC IIB, and BC III, than in the BN group, and the level in BC I and BC IIA was high enough to be distinguished from BN. Meanwhile, terminal fucosylation and high-mannose-type glycans appeared to be lowest in BC I. The glycosylation levels of BC I provide sensitivity and specificity that make BC I clearly distinguishable from BC IIA, BC IIB, and BC III as well as BN. Therefore, determination of serum AGP or AGP glycosylation level could be useful for detecting the early stages of breast cancer.

키워드

Alpha-1 acid glycoprotein; breast cancer; lectin; glycosylation

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