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Development of a Novel Short Synthetic Antibacterial Peptide Derived from the Swallowtail Butterfly Papilio xuthus Larvae

Journal of Microbiology and Biotechnology 2020년 30권 9호 p.1305 ~ 1309
김성률, 최광호, 김기영, 권혜용, 박성원,
소속 상세정보
김성률 ( Kim Seong-Ryul ) - RDA National Academy of Agricultural Science Sericultural and Apicultural Materials Division
최광호 ( Choi Kwang-Ho ) - RDA National Academy of Agricultural Science Sericultural and Apicultural Materials Division
김기영 ( Kim Kee-Young ) - RDA National Academy of Agricultural Science Sericultural and Apicultural Materials Division
권혜용 ( Kwon Hye-Yong ) - RDA National Academy of Agricultural Science Sericultural and Apicultural Materials Division
박성원 ( Park Seung-Won ) - Daegu Catholic University Department of Biomedical Science

Abstract


Insects possess biological defense systems that can effectively combat the invasion of external microorganisms and viruses, thereby supporting their survival in diverse environments. Antimicrobial peptides (AMPs) represent a fast-acting weapon against invading pathogens, including various bacterial or fungal strains. A 37-residue antimicrobial peptide, papiliocin, derived from the swallowtail butterfly Papilio xuthus larvae, showed significant antimicrobial activities against several human pathogenic bacterial and fungal strains. Jelleines, isolated as novel antibacterial peptides from the Royal Jelly (RJ) of bees, exhibit broad-spectrum protection against microbial infections. In this study, we developed a novel antimicrobial peptide, PAJE (RWKIFKKPFKISIHL-NH2), which is a hybrid peptide prepared by combining 1?7 amino acid residues (RWKIFKK-NH2) of papiliocin and 1?8 amino acid residues (PFKISIHL-NH2) of Jelleine-1 to alter length, charge distribution, net charge, volume, amphipaticity, and improve bacterial membrane interactions. This novel peptide exhibited increased hydrophobicity and net positive charge for binding effectively to the negatively charged membrane. PAJE demonstrated antimicrobial activity against both gram-negative and gram-positive bacteria, with very low toxicity to eukaryotic cells and an inexpensive process of synthesis. Collectively, these findings suggest that this novel peptide possesses great potential as an antimicrobial agent.

키워드

Antimicrobial peptide; hybrid peptide; papiliocin; Jelleine; biological safety

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